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scils lab software 2022a  (Bruker Corporation)


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    Structured Review

    Bruker Corporation scils lab software 2022a
    Scils Lab Software 2022a, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 97/100, based on 851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scils+lab+software+2022a/SCiLS+Lab/us12566180-451-41-45
    Average 97 stars, based on 851 article reviews
    scils lab software 2022a - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Produced:

    Article Title: Acute Lyme disease IgG N-linked glycans contrast the canonical inflammatory signature
    Article Snippet: In brief, 1μL serum diluted in 99μL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Lyme Disease IgG N-linked Glycans Contrast the Canonical Inflammatory Signature
    Article Snippet: In brief, serum samples incubated with 0.2μg/μl spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Acute lyme disease IgG N-linked glycans contrast the canonical inflammatory signature.
    Article Snippet: In brief, 1mL serum diluted in 99mL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release Nglycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Glycoproteomics:

    Article Title: Acute Lyme disease IgG N-linked glycans contrast the canonical inflammatory signature
    Article Snippet: In brief, 1μL serum diluted in 99μL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Lyme Disease IgG N-linked Glycans Contrast the Canonical Inflammatory Signature
    Article Snippet: In brief, serum samples incubated with 0.2μg/μl spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Acute lyme disease IgG N-linked glycans contrast the canonical inflammatory signature.
    Article Snippet: In brief, 1mL serum diluted in 99mL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release Nglycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Software:

    Article Title: Acute Lyme disease IgG N-linked glycans contrast the canonical inflammatory signature
    Article Snippet: In brief, 1μL serum diluted in 99μL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Lyme Disease IgG N-linked Glycans Contrast the Canonical Inflammatory Signature
    Article Snippet: In brief, serum samples incubated with 0.2μg/μl spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80-104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release N-glycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    Article Title: Acute lyme disease IgG N-linked glycans contrast the canonical inflammatory signature.
    Article Snippet: In brief, 1mL serum diluted in 99mL 1X PBS was incubated with 0.2mg/ml spotted anti-IgG capture antibodies (Bethyl Laboratories Inc., Tx, Cat. .. Number A80104) treated with Sialidase A (produced in-house by MUSC), sprayed with PNGase F (produced by N-Zyme, PA) to release Nglycans from captured targets, coated with a matrix, and analyzed for glycan abundance at specific m/z peaks by MALDI-FT-ICR MS using SCiLS Lab software 2022a (Bruker). ..

    other:

    Article Title: Methods of diagnosing or treating Lyme disease
    Article Snippet: MALDI-FT-ICR Mass Spectrometry N-Glycan Analysis SolarisX Legacy 7T FT-ICR mass spectrometer equipped with a Matrix-Assisted Laser Desorption/Ionization (MALDI) (Bruker) analysis of total IgG N-glycans is detailed in the literature.



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    Figure 7. Comparison of the spatial distribution of naloxone (sodium adduct ion (m/z 350.136) and potassium adduct ion (m/z 366.110)) inside ZF larval whole body between (a,b) the protocol used in the previous studies [26,27] and (c,d) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in Figure 4. The experimental conditions were the following: (a,b) 14 passes of spraying matrix deposition using 15 mg mL−1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; (c,d) 8 passes of spraying matrix deposition using 5 mg mL−1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
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    Image Search Results


    Figure 7. Comparison of the spatial distribution of naloxone (sodium adduct ion (m/z 350.136) and potassium adduct ion (m/z 366.110)) inside ZF larval whole body between (a,b) the protocol used in the previous studies [26,27] and (c,d) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in Figure 4. The experimental conditions were the following: (a,b) 14 passes of spraying matrix deposition using 15 mg mL−1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; (c,d) 8 passes of spraying matrix deposition using 5 mg mL−1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

    Journal: International journal of molecular sciences

    Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.

    doi: 10.3390/ijms241210076

    Figure Lengend Snippet: Figure 7. Comparison of the spatial distribution of naloxone (sodium adduct ion (m/z 350.136) and potassium adduct ion (m/z 366.110)) inside ZF larval whole body between (a,b) the protocol used in the previous studies [26,27] and (c,d) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in Figure 4. The experimental conditions were the following: (a,b) 14 passes of spraying matrix deposition using 15 mg mL−1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; (c,d) 8 passes of spraying matrix deposition using 5 mg mL−1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

    Article Snippet: 2.2.0, flexImaging version 5.0, and SCiLS Lab version 2022a Premium 3D software (Bruker Daltonics, Bremen, Germany).

    Techniques: Comparison, Software, Generated, Concentration Assay

    Figure 8. MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 (m/z 326.078), M4 (m/z 368.125), M7 (m/z 542.142), M10/M14 (m/z 382.105), and M35 (m/z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in Figure 4. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

    Journal: International journal of molecular sciences

    Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.

    doi: 10.3390/ijms241210076

    Figure Lengend Snippet: Figure 8. MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 (m/z 326.078), M4 (m/z 368.125), M7 (m/z 542.142), M10/M14 (m/z 382.105), and M35 (m/z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in Figure 4. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

    Article Snippet: 2.2.0, flexImaging version 5.0, and SCiLS Lab version 2022a Premium 3D software (Bruker Daltonics, Bremen, Germany).

    Techniques: Software, Generated, Concentration Assay